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What are the four general properties of transcriiption factors?
What are enhancers and how do they aid in transcriiption?
What are heterochromatin and euchromatin and what is their significance for transcriiption?
How does acetylation of histones affect gene expression in eukaryotes?
Describe the sequence of events needed to activate a eukaryotic gene?
What is gene silencing and What role does methylation play in silencing?
What are the consequences of not repairing DNA damage or the replication mechanism proceeding through damaged areas?
Which repair systems monitor the DNA double helix for damage rather than individual errors?
What kind of damage does the excision repair system fix?
How does the transcriiption-coupled excision repair system work?
Describe nonhomologous end joining?
Describe the differences between exonucleases and endonucleases.
Do restriction enzymes always cut the DNA at the recognition sequence? Describe the two major classes of restriction enzymes.
What two types of “end” do restriction enzymes produce? What is the difference between them?
Name four methods of detecting DNA.
What are the different steps involved in one cycle of a PCR reaction?
What are degenerate primers? Name two uses for degenerate primers.
What is reverse transcriiptase and how is it used in PCR?
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